Description
jMOTU
jMOTU is a super handy tool that makes it easy to cluster barcode DNA sequence data into molecular operational taxonomic units (MOTU). Not sure what a MOTU is? No worries! You can check out the DNA Barcoding pages on our website for more info.
What Can jMOTU Do?
So, what exactly can jMOTU do? Here’s a quick rundown:
- It reads input sequences in FASTA or NEXUS format.
- Calculates distances between pairs of sequences using a mix of BLAST and the Needleman-Wunch exact global alignment algorithm.
- Clusters input sequences into MOTUs using different cutoff measures.
Processing Large Datasets
jMOTU can handle big datasets like a champ! It uses various filtering steps to reduce the number of exact global alignments it needs to perform. When figuring out pairwise distances, it ignores gaps and only counts nucleotide mismatches. This makes it pretty tough against sequencing errors that might come from homopolymer runs!
The Clustering Process
The clustering happens with a greedy algorithm that doesn’t care about the order of your input sequences. In fact, jMOTU has been tested on raw datasets with about 50,000 input sequences! And if you have even larger datasets? No problem! It can cluster them too by first pre-clustering smaller subsets before combining everything for a thorough analysis.
Download jMOTU
If you’re ready to dive in and start clustering your DNA sequence data, don’t hesitate to get your hands on jMOTU!
User Reviews for jMOTU 6
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jMOTU is an exceptional app for DNA sequence analysis! It’s user-friendly and processes large datasets efficiently.
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I love how jMOTU simplifies the clustering of DNA sequences. The results are accurate, and it saves so much time!
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Fantastic tool for bioinformatics! jMOTU is easy to navigate and delivers reliable clustering outcomes quickly.
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Highly recommend jMOTU! It handles large datasets with ease and makes the MOTU process straightforward and efficient.
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jMOTU has transformed my research workflow. It's powerful yet simple to use, making DNA analysis a breeze!
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Amazing app! jMOTU's ability to cluster sequences accurately while minimizing errors is a game changer in DNA research.