Oligo

Oligo is a must-have tool for anyone working with DNA. It helps you design and analyze sequencing and PCR primers, synthetic genes, and all sorts of probes like siRNA and molecular beacons. Whether you're a student or a pro in genetics, Oligo makes things simpler!

Smart Primer Design

This software uses the latest nearest neighbor thermodynamic data to find the best primers for PCR. It can handle TaqMan, multiplexed setups, consensus primers, or even degenerate ones. Plus, you can process multiple files at once! It's also super handy for site-directed mutagenesis.

Detailed Analysis Windows

With Oligo, every primer pair gets its own analysis window packed with useful info. You can see DNA and RNA secondary structures, check for dimer formation, false priming issues, homology data, internal stability, composition details, and physical properties—all in one spot.

Protein Insights

You can dive deeper into open reading frames to predict molecular weights and pKa values of proteins. Oligo even helps you search for restriction enzyme sites in both DNA and reverse-translated proteins!

Automatic Features

The cool part? Oligo can automatically select multiplex primers! Need to process sequence files quickly? No problem! It designs PCR primers that cover multiple DNA regions in just one go. And if you're running real-time PCR? It'll find the perfect primer/probe sets or nested primer sets automatically.

Customized Search Protocols

You also get to customize your search protocols by adjusting the scoring system. This means you can optimize results based on your specific needs—how great is that?

If you're interested in checking out more about Oligo, head over to Softpas for all the details!