PrimerPlex is a super handy software that helps you analyze and design multiplex oligos platforms for ASPE and direct hybridization assays. It's like having a smart assistant that checks each probe for cross homologies, making sure you get strong signals. Plus, you can screen oligos for dimers, runs, and repeats without breaking a sweat!
This software lets you view sequences in separate tabs, all organized into sequence info, search status, and multiplex assay types. In the Sequence Information tab, you can dive into details like sequence length and temperature while keeping an eye on dimer or cross-dimer issues. The Search Status display shows off multiple properties at once.
You can pick one or more entries from the sequence database to compare their primer properties. Want to check out capture probe attributes or mutations? No problem! You can even see nucleobase sequences laid out nicely.
The Multiplexed Assays tab is where things get interesting! Here, you can check the rating and status of each capture probe along with the results from your multiplex designs.
PrimerPlex shines when it comes to designing multiplex primer sets. This means combining multiple primers in one assay to see how they interact. With multiplex assays, you can amplify oligos in a single reaction vessel, speeding up your research while saving some bucks!
You'll find it easy to whip up multiplex PCR primers or composition for hybridization assays and ASPE assays using this software. It employs complex design algorithms based on combinations and natural patterns to show how specific combinations work together.
The software is capable of designing standard primers for a 30-plex multiplex PCR assay and screening optimal primer sets under uniform reaction contexts for up to 30 targets!
PrimerPlex is an invaluable assistant in genome mutation research. It allows you to design various types of analyses like multiplex primer assays and hybridization assays easily! You can create cool nucleobase sequences combinations too! If you're ready to give it a try, check it out here!
Go to the Softpas website, press the 'Downloads' button, and pick the app you want to download and install—easy and fast!
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