X2XML is a free and open-source tool that helps you convert files containing mass spectrometry data into the mzXML file format. To get started, you'll need to have the right vendor software already installed on your computer.
This lightweight program works as an extension of the ReAdW tool and supports various file types like Xcalibur .raw, Agilent .wiff, Micromass .dat (found inside .raw folders), Bruker .acqu, Bruker .ascii, PNNL ion-mobility spectrometry (IMS) .imf, and even Bruker FTICR S-folders. The last one is a neat way to represent large S files made by Bruker FTICR as a single-file per spectrum!
Before you set up X2XML, make sure you have .NET Framework 1.1 installed on your machine. The interface is pretty straightforward—it has a standard window where you can see all your options.
You can easily choose the S and IMS folders for your data. Just select the file with the mass spectrometry data you'd like to convert, edit the dataset name if needed, and pick your preferred dataset type.
Plus, there’s a handy feature that lets you set minimum and maximum values for the m/z filter range or scan range. This helps you exclude very small or large m/z values so your output file isn’t too big. With just one click, you can convert the file or unzip it before conversion!
Just a heads up: this program isn't supported anymore and won't work on operating systems newer than Windows Vista. Also, keep in mind that it only works with .NET Framework 1.1, so newer versions won’t be compatible.
Go to the Softpas website, press the 'Downloads' button, and pick the app you want to download and install—easy and fast!
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